Study of the Effect of Innate on the Inflammatory Response to Endotoxin

• INCLUSION CRITERIA:
• Male or female 18 years of age or older
• Participants must be able to understand and provide written informed consent to participate in the study
• Participants must be able to travel to the CRU
• Willing and able to fast after midnight the night prior to their study appointment.
• Healthy participants as defined by the International Red Cross guidelines (Healthy means that an individual feels well and can perform normal activities. If the individual has a chronic condition such as diabetes or high blood pressure, healthy also means that they are being treated and the condition is under control).

EXCLUSION CRITERIA

• Use of nonsteroidal anti-inflammatory drugs (NSAIDs) within 5 days prior to enrollment visit (e.g., Motrin, ibuprofen, naproxen, and Advil)
• Use of acetaminophen (Tylenol) within 5 days prior to enrollment visit
• Use of cholesterol lowering drugs (statins) within 30 days prior to enrollment visit (e.g., Zocor, Mevacor, Lipitor, and Crestor)
• Use of immunosuppressants or other immune-modifying drugs [e.g., Rituxan, Humira, Enbrel, Cyclosporin (Neoral, Sandimmune, and SangCya), and Azathioprine (Imuran)], Monoclonal antibodies [e.g., infliximab (Remicade)], and corticosteroids (e.g., prednisone, prednisolone and dexamethasone)
• Current treatment for cancer with chemotherapy or radiation
• Confirmed or suspected immunosuppressive or immunodeficient condition
• GI or respiratory Illness within 5 days prior to enrollment visit, including cold or allergies
• Smoked tobacco, chewed tobacco or used electronic cigarettes within 2 weeks prior to enrollment visit (for participants who provide a urine specimen, this will be defined by urine cotinine >200 ng/mL at visit)
• Alcohol consumption greater than 2 standard drinks (1 standard drink contains 15 g of ethanol) per day within the last 24 hours prior to the enrollment visit
• Body weight \< 50 kg (\<110 lbs)
• Temperature > 37.6 C; blood pressure \< 90/50 mm Hg or > 170/95 mm Hg; pulse rate \< 50 or >100 beats/minute
• Pregnant or suspected pregnancy
• Chronic Kidney Disease

The PI may review medication use on a case by case basis and make a medical determination on the participant s eligibility. In these cases, the PI determination will be documented in the participant s chart.